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Biotinylated GPX4 protein prepared by Thermo EZ-Link long-chain biotinylation reagent was connected to a Super Streptavidin (SSA) biosensor by immersing the sensor in a 96-well black plate loaded with protein solution, the concentration of which was predetermined by preliminary control experiments to achieve the best signal-to-noise ratio
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10.1016/0016-5085(93)90895-J 87
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