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On the other hand, transfection of individual mu-ARE reporter plasmids in which the consensus ARE was mutated failed to induce luciferase activity in Keap −/− MEFs, and it was comparable to that in Nrf2 −/− cells and or vector alone, indicating the specificity of the “ARE motifs” in the induction of reporter activity (Fig
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The working principle is shown in Figure 5
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