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Desalted peptides were separated on an EASY-Spray PepMap C18 column (50 cm × 50 μm ID, 2 μm particles, 100 Å pore size) using a linear gradient of 5% to 35% buffer B (0.5% formic acid in 80% acetonitrile)

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Size:UK6 / EU39 / US8 / AU8 - WIDE FIT

Gene Expression Analysis by RT-qPCR Purified RNA was treated with DNAse I, RNase-free (Thermo Scientific™, Waltham, MA, USA) according to the manufacturer’s protocol, and reverse transcribed into cDNA using the iScript™ Reverse Transcription Supermix (Bio-Rad Laboratories, Mississauga, ON, Canada)

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