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2.4.2 Histological analysis Animal tendons were fixed in a 10% neutral formaldehyde solution for at least 48 h

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Observed responses in research settings are generally reported as mild and may include: Temporary injection-site irritation Redness or warmth sensations Skin-sensitivity variability Temporary blue-tinted discoloration beneath the administration area Localized irritation depending on dosage range, concentration, administration frequency, protocol duration, and individual sensitivity Research protocols commonly investigate daily administration ranges between: 1–2mg daily in standard research settings Up to 3mg daily in more advanced experimental protocols GHK-Cu is frequently explored in continuous or maintenance-style research structures and does not always require strict cycling protocols in moderate-dose experimental models

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