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Briefly, various concentrations (10–1000 μg/mL) of selected compounds were mixed with the mh-Tyr (2000 U/mL) and 5X sample buffer [1.5 M Tris–HCl (pH 6.8), 10% glycerol, and 0.01% bromophenol blue] followed by incubation on the ice for 30 min
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