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Homogenates were centrifuged at 5000 g for 20 min, and the supernatants used to determine the amount of MDA by preparing a solution containing 250 μL of homogenate, 100 μL of 8% sodium dodecyl sulfate (SDS), 750 μL of 20% acetic acid, 750 μL of 0.08% TBA, and 150 μL of distilled water, which was pipetted into capped test tubes and vortexed

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